Figure 2.

Cytokine expression in MSC and monocytes in the co-cultures. Cytokine protein levels in the supernatant of monocyte cultures at day 6 for IL-10 (A) and IL-6 (B) in the presence or absence of MSC (IL-10: mean±SEM of three independent experiments with three different monocyte donors and six different MSC donors; IL-6: mean of six independent experiments with six different monocyte donors and seven different MSC donors; *P<0.05, **P<0.01, ***P<0.001). (C) IL-6 and IL-10 mRNA expression in MSC after 6 days of culture with and without monocytes and growth factors in a transwell co-culture system. Fold change is relative to MSC alone. (D) IL-6 and IL-10 mRNA expression in the monocyte-derived cell population from the transwell co-culture at day 6 of differentiation in the absence or presence of MSC. Fold change is relative to MDC differentiated in the absence of MSC. (E) IL-6 secretion of MSC after 2 days of culture in fresh medium following 6 days of culture with or without growth factors and monocytes. (F) IL-6 secretion of monocyte-derived-cells (MDC) after 2 days of culture in fresh medium following differentiation in the absence or presence of MSC. (C-F: data are mean±SEM of three different MSC donors, *P<0.05, ** P<0.01, ***P<0.001).