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. 2013 Jun;98(6):888–895. doi: 10.3324/haematol.2012.078055

Figure 4.

Figure 4.

IL-6, but not IL-10, is directly involved in the inhibitory effect of MSC on monocyte differentiation. The role of IL-10 and IL-6 in the MSC-mediated inhibition of monocyte differentiation was investigated. (A) Adding a neutralizing antibody against IL-10 (aIL-10) did not restore iDC formation (data are representative dotplots from two independent experiments with two different MSC donors and two different monocyte donors). (B, C) The addition of a neutralizing antibody against IL-6 (aIL-6) (2.5 μg/mL) to the transwell co-culture significantly reduced the inhibitory effect of MSC (data are from three independent experiments with four different MSC donors and four different monocyte donors. Statistical analysis was performed using the Wilcoxon signed rank test *P<0.05). (D, E) The increase of IL-6 and IL-10 mRNA expression on day 6 monocytes (fold change is relative to iDC) (D) and protein secretion in the co-culture on day 6 (E) was reversed when an IL-6 neutralizing antibody was added to the culture (data are means±SD of two different MSC donors and one monocyte donor; * P<0.05).