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. 2013 Jun;98(6):888–895. doi: 10.3324/haematol.2012.078055

Figure 5.

Figure 5.

IL-6 is the key factor in the MSC-induced inhibition of monocyte differentiation. IL-6 (A) and IL-10 (B) were added to the monocyte differentiation cultures. Both IL-6 and IL-10 inhibited the differentiation to iDC in a manner similar to the effect of co-culture with MSC/MSC-CM and addition of IL-6 directly increased the IL-10 secretion by monocyte-derived cells (A) (data are means ± SD from two different MSC; *P<0.05). (C) Depletion of IL-6 from the MSC conditioned medium partly reversed the inhibiting effect of MSC on the monocyte-derived iDC differentiation (representative dotplot of three different MSC donors and two different monocyte donors. (D) MSC-derived IL-6 drives the IL-10 upregulation in the monocyte-derived cell population (C, D: data are means±SD from two different MSC; ***P<0.001). (E) Corrected counts per minute (CCPM) of iDC and day 6 MDC from cultures in MSC-CM and IL-6-depleted MSC-CM that were incubated with CD4+CD25− T cells to assess their allostimulatory capacity. Data are from two different MSC donors and the experiment was performed in triplicate; significance is relative to iDC (*P<0.05, **P<0.01, ***P<0.001).