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. 2013 Jun;98(6):918–927. doi: 10.3324/haematol.2012.074195

Figure 5.

Figure 5.

EZH2 interacts with C/EBPα in MA9 cells and represses C/EBPα target genes. (A) Luciferase assay in HEK 293T cells with a C/EBPα binding site-containing promoter-driven luciferase plasmid, C/EBPα and increasing amounts of EZH2. (B) Luciferase assay in HEK 293T cells with a Gli-1 binding site-containing promoter-driven luciferase plasmid, Gli-1 and increasing amounts of EZH2. (C) Luciferase assay in RAW264.7 cells with a C/EBPα binding site-containing promoter-driven luciferase plasmid, C/EBPα and increasing amounts of EZH2. (D) Immunoprecipitation (IP) for C/EBPα followed by western blotting for EZH2 (top) or C/EBPα (bottom). (E) IP for EZH2 followed by western blotting for C/EBPα (top) and EZH2 (bottom) in THP-1 cells.