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. 2013 Jun;98(6):918–927. doi: 10.3324/haematol.2012.074195

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Figure 6. — EZH2 knockdown induces MA9 cell differentiation. (A) ChIP assay for EZH2 enrichment at the Mcsfr, Id2, and Pparg loci in control or Men1-excised AT-1 cells 6 days after 4-OHT treatment. (B) ChIP assay for H3K27m3 enrichment at the Mcsfr promoter in control or Men1-excised AT-1 cells 6 days after 4-OHT treatment. (C) Western blot for EZH2 expression in Scr control and EZH2 knockdown THP-1 cells. (D) Real-time PCR analysis of Scr control and EZH2 KD THP-1 cells for C/EBPα target genes. (E) Flow cytometry analysis of CD11b cell surface expression in Scr and EZH2 knockdown THP-1 cells. (F) A model for the role of EZH2 in MA9 leukemia.