FIG. 2.

Detection of tick-borne encephalitis virus (TBEV)-specific antigen and RNA. Baby hamster kidney (BHK) cells were inoculated with the brain homogenate of a sick mouse inoculated with the spleen homogenate of wild rodents. Inoculated (A) and mock-treated (B) cells were stained using anti-tick-borne flavivirus antibodies. (C) TBEV-specific products were amplified by RT-PCR from cells infected with the brain homogenate of the sick mouse (lane 1). TBEV-infected (lane 2) and mock-treated (lane 3) cells were used as positive and negative controls, respectively.