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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cancer Chemother Pharmacol. 2013 Apr 10;71(6):1567–1576. doi: 10.1007/s00280-013-2156-3

Fig 2. Effects of calpain inhibition on caspase activity in bortezomib induced cell death.

Fig 2

Myeloma cells were incubated with the indicated concentration of bortezomib in the presence or absence of 10 nM PD150606 or 10 μM Calpain Inhibitor IV for 24 hours. Cells were lysed and assayed for caspase-3 (A), caspase-8 (B) and caspase-9 (C) activity using pNA labeled tetrapeptides. Data shown are the mean of three independent experiments ± SE. * significant vs. BZ only at p < 0.05. (D) Myeloma cells were pretreated with the pan caspase inhibitor Q-VD-OPH for 30 minutes prior to drug treatment and analyzed for cell death by Annexin V/PI staining and flow cytometry. Values are the mean of 3 independent experiments, and data is presented as [(treated−%control)/(100−control)] where the control is calpain inhibitor only.