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Increased pro-mitogenic gene expression in HNF4α-KO tumors. (A) Western blot analysis of HNF4α, Cyclin D1, and c-Myc using either nuclear proteins (HNF4α) or total liver extracts (all other) of Control mice, Control mice treated with DEN, normal liver tissue of HNF4α-KO treated with DEN and tumor tissue of HNF4α-KO treated with DEN. (B) to (G) Real time PCR analysis of putative negative targets of HNF4α identified by combined RNA-seq-ChIP-seq bioinformatics analysis.
