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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Endocr Relat Cancer. 2013 May 30;20(3):415–429. doi: 10.1530/ERC-12-0005

Figure 2. TRPC3 knockdown attenuated the effects of FSH on proliferation in ovarian cancer cells.

Figure 2

(A and B) Knockdown of TRPC3 expression by TRPC3 siRNA (siTRPC3). (A) A representative Western blot of HEY and ES-2 cells is shown. The cells were transfected with siTRPC3, the total lysates were extracted, and the immunoblots were probed with anti-TRPC3 and GAPDH antibodies. The transfectants without siRNA and with non-targeting control siRNA (siCon) were used as controls. (B) A semi-quantitative analysis of the TRPC3/GAPDH ratio was performed. The data represent the mean ± SD of three independent Western blot assays as in (A). *P<0.05, compared with siCon control. (C, D) The cell growth stimulation effects of FSH (by different concentration) were reduced by TRPC3 knockdown in HEY (C) and ES-2 (D) cells. The cells were transfected with siTRPC3 and treated with FSH at various concentrations ranging from 0 to 80 mIU/ml for 48 hr. The siCon transfectants were used as a control. The cell proliferation rate was detected using the SRB assay. Each experiment was performed in triplicate. *P<0.05, compared with siCon transfectants. (E, F) The cell growth stimulation effects of 40 mIU/ml FSH (at different stimulation times of 0 to 72 h) were reduced by TRPC3 knockdown in HEY (E) and ES-2 (F) cells. *P<0.05, compared with siCon transfectants. (G, H) The cell cycle changes induced by FSH stimulation were attenuated by TRPC3 knockdown in HEY (G) and ES-2 (H) cells. The cells were transfected with siTRPC3 and either treated with FSH at 40 mIU/ml or left untreated for 48 hr. The cell cycle distribution was measured using flow cytometry and is displayed as a proliferation index. The data represent the mean ± SD of three independent assays. *P<0.05 or **P<0.01, compared with siCon control.