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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Mutat Res. 2013 Mar 13;0:46–54. doi: 10.1016/j.mrfmmm.2013.03.001

Figure 4. Error rate when copying a cis-syn cyclobutane thymine-thymine dimer by pol η mutants.

Figure 4

Values given are calculated as described in the Materials and Methods and represent errors per 10,000 bases copied. Bypass fidelity reactions used a 2:1 substrate:polymerase ratio and were incubated at 37 °C for 30 minutes. Values come from sequencing (for each form) 83–135 dark blue plaques for undamaged DNA and 38–52 dark blue plaques for T-T CPD reactions, with each plaque representing a unique bypass event. A. Values for T→C errors (dGTP misinsertion) opposite the 3′T of either undamaged (white bars) or damaged (hatched bars) template DNA. B. Values for T→A errors (dTTP misinsertion) opposite the 3′T of either undamaged (white bars) or damaged (hatched bars) template DNA. In both panels the values for wild type pol η are consistent with values generated using full length pol η in the same assay [6].