Table 2.
Base substitution and insertion/deletion error rates of pol η mutants copying undamaged DNA in a gapped plasmid
| Mutant | Error rate (× 10−4) * | ||||
|---|---|---|---|---|---|
| Single base substitution | Single base deletion | Single base insertion | Complex** | Tandem base substitution | |
| WT | 230 | 25 | 3 | 35 | 13 |
| M14V | 170 (0.7) | 9 (0.4) | 5 (1.8) | 22 (0.6) | 9 (0.7) |
| Q38A | 260 (1.1) | 18 (0.7) | 16 (5.3) | 100 (2.8) | 43 (3.3) |
| Q38V | 220 (1.0) | 12 (0.5) | 9 (3.1) | 46 (1.3) | 10 (0.8) |
| Y52E | 73 (0.3) | 10 (0.4) | ≤1 (0.5) | 3 (0.1) | 1 (0.1) |
| R61A | 74 (0.3) | 7 (0.3) | 4 (1.4) | 7 (0.2) | 1 (0.1) |
| S62A | 170 (0.7) | 11 (0.4) | 5 (1.8) | 16 (0.5) | 8 (0.6) |
| S62G | 170 (0.7) | 14 (0.6) | 3 (1.0) | 20 (0.6) | 8 (0.6) |
| R81C | 180 (0.8) | 21 (0.9) | 7 (2.3) | 36 (1.0) | 9 (0.7) |
| E82D | 120 (0.5) | 13 (0.5) | 8 (2.6) | 20 (0.6) | 5 (0.4) |
*
Values given correspond to errors per 10,000 bases copied.
**
Complex errors are defined as multiple changes occurring within 3 bases, such as substitution-deletions and substitution-insertions. The tandem base substitutions error rates shown are a subset of the Complex errors reported. Values in parentheses for mutants are relative to WT rate.