Fig. 8.
Substrate composition and laser spot dependence of interference artifacts in the optical lever arm deflection signal. (A) Force-extension curves taken on mica (black) and on gold (grey) for different positions of the laser spot on an uncoated long BioLever. Only the approach portion of the force curve is shown for clarity. (B) Optical microscope image of a long uncoated BioLever showing the laser spot position and defining x as the distance of the center of the laser spot from the reference point (x = 0 µm).