Table 1. Cytotoxicity assessed by LDH assay.
Values represent means, expressed as a percentage of total LDH activity induced by 1% Triton X-100. Supernatants of vehicle- and ligand- treated GPR55E U2OS cells are similar to each other in that they demonstrate little to no cellular damage, 7% and 6–9% of LDH activity, respectively.
| Treatment | Cell death, % |
|---|---|
|
| |
| HBSS | 5.97 |
| Vehicle | 7.12 |
| 3μM LPI | 7.08 |
| 30 μM SR141716A | 7.12 |
| 10 μM Virodhamine | 7.27 |
| 10 μM Virodhamine +3 μM LPI | 6.94 |
| 10 μM Virodhamine + 30 μM SR141716A | 6.44 |
| 30 μM Virodhamine | 8.08 |
| 30 μM Virodhamine + 3μM LPI | 8.73 |
| 30 μM Virodhamine +30 μM SR141716A | 6.93 |
| 10 μM AEA | 5.90 |
| 10 μM AEA+ 3μM LPI | 6.12 |
| 10 μM AEA + 30 μM SR141716A | 6.79 |
| 30 μM AEA | 5.93 |
| 30 μM AEA + 3 μM LPI | 5.86 |
| 30 μM AEA + 30 μM SR141716A | 8.02 |