Table 1.

Sample locations and aqueous geochemical parameters¹ of six, high-temperature phototrophic microbial communities sampled in Yellowstone National Park (YNP) and used for metagenome sequencing.

Location	T (°C)	pH	Na+	Cl−	${\text{SO}}_{\text{4}}^{\text{2-}}$	1DIC	1DS	1DO	1DOC	As	2Fe	3${\text{NH}}_{\text{4}}^{\text{+}}$	Coordinates
			mM				μM
Bath Lake Vista Annex-Green (BVLA_5)	57	6.2	3.9	4.4	5.6	15.8	117	<3	104	24	0.7	40	44° 57′54.180″N 110° 42′42.228″W
Bath Lake Vista Annex-Purple (BLVA_20)	54	6.2	5.5	5.7	7.3	24.2	117	<3	75	23	0.7	40	44° 57′54.180″N 110° 42′42.228″W
White Creek (WC_6)	52	8.2	3.6	1.8	0.23	nd	<3	188	nd	5	1.7	1.9	44° 31′53.399″N 110° 47′51.799″W
Chocolate Pots (CP_7)	52	6.2	4.1	0.89	0.23	13.2	<3	<3	38	9	75.5	4.2	44° 42′36.288″N 110° 44′28.824″W
Mushroom Spring (MS_15)	60	8.2	12.6	7.3	0.18	2.1	<3	141	nd	26	<1	4.4	44° 32′19.284″N 110° 47′52.692″W
Fairy Geyser (FG_16)	36–38	9.1	9.4	5.2	0.18	4.8	<3	31	30	13	<1	1.3	44° 32′31.812″N 110° 51′40.788″W
4Correlation (r2)		0.89*		0.93*	0.96*		0.99***				0.72**	0.99**

¹DS, total dissolved sulfide; DO, dissolved oxygen; DIC, dissolved inorganic carbon; DOC, dissolved organic carbon.

²Mn (total soluble) values were also significant in CP_7 (24 μM) and WC_6 (5 μM), but low in other sites (0.1–0.2 μM, or below detection of 0.1 μM).

³Nitrate values ranged from 2.1–6.7 μM across sites.

⁴Correlation significance values: *p < 0.05, **p < 0.01, ***p < 0.001.