Table 2.

Properties of scaffold clusters obtained from metagenome assemblies as demarcated with oligonucleotide composition and confirmed using phylogenetic analyses.

Scaffold cluster	Taxonomic affiliation	Sites	No. of scaffolds	Median size (kbase)	G+C (%) ± SD	Total sequence (Mbase)	Depth of coverage (x)
1	Roseiflexus spp.	BLVA_5, CP_7, MS_15, FG_16	112	12.5	60.0 ± 1.2	1.55	2.6 ± 0.4
2	Chloroflexus spp.	BLVA_5, BLVA_20, WC_6, CP_7	211	13.5	54.3 ± 1.2	3.21	2.9 ± 0.7
3	Ca. Thermochlorobacter spp.	CP_7	73	14.8	49.5 ± 0.8	1.13	2.7 ± 0.5
4	Thermochromatium spp.	BLVA_20	29	12.5	63.0 ± 1.3	0.37	2.1 ± 0.4
5	Synechococcus spp.	WC_6, CP_7	78	26.2	58.7 ± 1.1	2.59	4.0 ± 0.7
6	Cyanobacteria	WC_6, CP_7	26	11.7	49.8 ± 1.2	0.32	2.4 ± 0.5
7	Bacteroidetes	WC_6	30	11.1	37.7 ± 0.9	0.37	2.4 ± 0.4
8	Chloroflexi-like	BLVA_5, MS_15, BLVA_20	37	10.6	63.9 ± 2.3	0.44	2.5 ± 0.5
9	Firmicute-like	BLVA_5, CP_7, BLVA_20	47	14.2	36.0 ± 1.5	0.79	2.7 ± 0.4
10	Firmicute-like	BVLA_5, BLVA_20	11	12.7	29.0 ± 1.4	0.16	2.6 ± 0.6
11	Spirochaetes	CP_7	21	11.8	30.5 ± 1.4	0.25	2.3 ± 0.4

Scaffold clusters 7–11 represent novel bacteria that are not well represented in public databases, and are currently defined at the phylum level.