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Effects of cisplatin on mRNA expression of β-tubulin, CD49f, and SSEA4 in breast cancer cells. Individual PCR reactions were normalized against internal positive control (β-actin) and plotted as the mRNA relative expression for both untreated and cisplatin-treated BT-549 (A) and MDA-MB-231 (B) breast cancer cells. Cells were incubated with 20 μM cisplatin for 24 h. Experiments were done in duplicates in three independent experiments. Bars are presented as mean ± SEM. *p ≤ 0.05.
