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. 2013 Jun 3;3:134. doi: 10.3389/fonc.2013.00134

Figure 6.

Figure 6

Comparison of cisplatin effect on mRNA expression of CD49f, a stem cell marker, at low and high cisplatin doses in breast cancer cells. Individual PCR reactions were normalized against internal positive control (β-actin) and plotted as the mRNA relative expression for both untreated and cisplatin-treated BT-549 and MDA-MB-231 breast cancer cells. Cells were incubated with 5 and 20 μM cisplatin for 24 h. Experiments were done in duplicates in three independent experiments. Bars are presented as mean ± SEM. *p ≤ 0.05.