Table 2.
Antibodies used.
| Antibody | Clone | Cat. number | Company | Application |
|---|---|---|---|---|
| Nestin | 3k1 | 09-0045 | Stemgent | FC: 1:50 |
| CK18 | CY90 | MCA1864H | AbD Serotec | FC: 100 μL, IF: 100 μL |
| Smooth muscle actin (SMA) | CGA7 | Sc-53015 | Santa Cruz Biotechnology, Inc. | FC: 1:50, IF: 1:10 |
| β-Tubulin | – | Hybridoma | FC: 100 μL, IF: 100 μL | |
| Stage specific embryonic antigen-4 (SSEA4) | MC-813-70 | 09-0006 | Stemgent | FC: 1:50 |
| Integrin-α6 (CD49f) | GoH3 | 555735 | BD Pharmingen™ | FC: 1:4 |
| Alexa Fluor 488 donkey anti-mouse IgG (H + L) | – | A-21202 | Invitrogen, USA | FC: 1:50, IF: 1:100 |
| Alexa Fluor 488 donkey anti-rabbit IgG (H + L) | – | A-21206 | Invitrogen, USA | FC:1:50 |
| FITC rat IgG2a.k | R35-95 | 555843 | BD Pharmingen™ | FC:1:4 |