Table 3.
Gene | Forward primer | Reverse primer | Annealing temperature (°C) |
---|---|---|---|
SSEA4 | 5′TGG ACG GGC ACA ACT TCA TC 3′ |
5′GGG CAG GTT CTT GGC ACT CT 3′ |
54 |
CD49f | 5′ATG GAG GAA ACC CTG TGG CT 3′ |
5′ACG AGA GCT TGG CTC TTG GA 3′ |
60 |
β-Tubulin 6 | 5′AGG CTA CGT GGG AGA CTC G 3′ |
5′GCC CTG GGC ACA TAT TTC T 3′ |
60 |
β-Actin | 5′CGG CAT TCA CGA AAC 3′ |
5′GGG CAG TGA TCT CTT 3′ |
55 |