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. 2013 Jun 3;3:134. doi: 10.3389/fonc.2013.00134

Table 3.

Primers used for RT-PCR.

Gene Forward primer Reverse primer Annealing temperature (°C)
SSEA4 5′TGG ACG GGC
ACA ACT TCA TC 3′
5′GGG CAG GTT
CTT GGC ACT CT 3′
54
CD49f 5′ATG GAG GAA
ACC CTG TGG CT 3′
5′ACG AGA GCT
TGG CTC TTG GA 3′
60
β-Tubulin 6 5′AGG CTA CGT
GGG AGA CTC G 3′
5′GCC CTG GGC
ACA TAT TTC T 3′
60
β-Actin 5′CGG CAT TCA
CGA AAC 3′
5′GGG CAG TGA
TCT CTT 3′
55