Table 4.
Breast cancer cell characterization and effects of cisplatin on protein expression.
| Cell line | Cell type | Markers | No drug (MFI) | Cisplatin (MFI) | Up/down-regulation (%) |
|---|---|---|---|---|---|
| BT-549 | Stem cells | SSEA4 | 22.2 | 7.2 | ↓(67.6) |
| CD49f | 60 | 27 | ↓(54.8) | ||
| Progenitor cells | Nestin | 45 | 21 | ↓(52.3) | |
| Differentiated cells | SMA | 7.8 | 17.9 | ↑(129.5) | |
| CK18 | 7 | 2 | ↓(71.2) | ||
| β-Tubulin | 66 | 148.6 | ↑(125.2) | ||
| MDA-MB-231 | Stem cells | SSEA4 | 518.2 | 205.3 | ↓(60.4) |
| CD49f | 53 | 24 | ↓(55) | ||
| Progenitor cells | Nestin | 35.8 | 45.5 | ↑(27) | |
| Differentiated cells | SMA | 9.7 | 6.2 | ↓(36) | |
| CK18 | 41.2 | 45.4 | ↑(10.2) | ||
| β-Tubulin | 49.6 | 83.6 | ↑(68.5) | ||
| MDA-MB-468 | Stem cells | SSEA4 | 6.5 | 4 | ↓(38.5) |
| CD49f | 27 | 15 | ↓(44.4) | ||
| Progenitor cells | Nestin | 81 | 65 | ↓(19.6) | |
| Differentiated cells | SMA | 25 | 13 | ↓(48) | |
| CK18 | 161.8 | 262.4 | ↑(62.2) | ||
| β-Tubulin | 52.9 | 82 | ↑(55) | ||
| MCF-7 | Stem cells | SSEA4 | 8.02 | 7.02 | ↓(12.5) |
| CD49F | 27.2 | 15.8 | ↓(41.9) | ||
| Progenitor cells | Nestin | 30.3 | 29.4 | ↓(3) | |
| Differentiated cells | SMA | 14.2 | 10.2 | ↓(28.2) | |
| CK18 | 173.1 | 224 | ↑(29.4) | ||
| β-Tubulin | 74.9 | 141 | ↑(88.3) |
MFI, mean fluorescence intensity; ESC, embryonic stem cell; SSEA4, stage specific embryonic antigen-4; CD49f, integrin subunit-α6; SMA, smooth muscle actin; CK18, cytokeratin 18.