Figure 4.

Chromatin immunoprecipitation (ChIP) experiments were performed for transcription factors (TF ChIP) and histone modifications (HM ChIP) in Flinders resistant line (FRL) and Flinders sensitive line (FSL). (a) TF ChIP at the rs105431668-containing (target) region showed an enrichment of CREB2, Ep300, and acetylated Ep300 only in the FRL. (b) As Ep300 is one of the main histone acetyltransferases (HATs) necessary for in vivo maintenance of H3K18ac, we performed HM ChIP against H3K18ac of Npy. In line with Ep300's HAT activity, the HM ChIP showed increased levels of H3K18ac in FRL compared with FSL. *P<0.05, **P<0.01.