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. 2013 May 21;3(5):e259. doi: 10.1038/tp.2013.35

Figure 3.

Figure 3

EAAC1 isoforms inhibit glutamate transport from the EAAC1 transporter.(a) EAAC1 isoforms inhibit endogenous glutamate uptake in HEK-293 cells. Normalized glutamate uptake (30 or 60 min) is depicted in HEK-293 cells transfected with 60 ng of the indicated DNA (the primary transcript of EAAC1 (P1), with each of the EAAC1 isoforms (P2, ex2skip or ex11skip) or GTRAP3-18), non-transfected cells (NT) or cells treated with the drug, DL-TBOA, which inhibits active glutamate transport. Results±s.e.m. are shown for triplicate assays conducted in two independent experiments (N⩾6). *P⩽0.05 and **P⩽0.01 in comparison to the pcDNA3.1 control (unpaired two-tailed t-test). (b) EAAC1 isoforms inhibit exogenous glutamate uptake derived from the transfected P1 transcript in HEK-293 cells. HEK-293 cells were transfected with 10 ng P1 (in each well of a 96-well plate) and increasing amounts of the indicated other DNA. The total amount of DNA in each well was adjusted to 60 ng using pcDNA3.1. Exogenous glutamate uptake was normalized using 100% as the maximal uptake observed with 10 ng P1 and 0% as the uptake observed with only pcDNA3.1. Results±s.e.m. are shown for triplicate assays conducted in two independent experiments (N⩾6) after 30 or 60 min of glutamate uptake. *P⩽0.05 and **P⩽0.01 in comparison to maximal uptake in the absence of competitor (unpaired two-tailed t-test). eGFP, enhanced green fluorescent protein.