Fig. 3.

Sigma1R co-expression had no effect on the sensitivity of Kv1.3 inhibition by Sigma1R ligands. A. Time course of ketamine inhibition of peak currents in oocytes expressing Kv1.3 alone (closed circles) or co-expressing Sigma1R (open circles). Currents were evoked by repetitive 800 ms voltage steps from −80 to 0 mV every 15 seconds to circumvent accumulation of cumulative slow inactivation. Ketamine (1 mM) was administrated at the rate of 1 ml/min by gravity. The current waveforms recorded at different time points during the experiment (Kv1.3 only) are shown below. When administrated in the same way, ketamine (B), haloperidol (C) and (+)SKF 10047 (D), all showed a dose-dependent current inhibition of the Kv1.3 peak current. n=3 for each ligand. E. The upper traces are from before and during drug application. The same traces were scaled and overlapped demonstrating no change in current kinetics induced by ketamine, haloperidol (F) and (+)SKF 10047 (G).