Figure 5. Loss of αTAT activity reveals lattice defects and variations in protofilament number in individual microtubules.
(A, B) Example of a microtubule lattice defect associated with a change in protofilament number on opposite sides of the defect (10 vs. 13).
(C, D) Example of a microtubule lattice defect with no change in protofilament number. Images were selected from two serial-section reconstructions consisting of seventeen (17) consecutive thin (50-nm) sections. The datasets were used to reconstruct two TRN microtubules in mec-17;atat-2 double mutants.
