TABLE 1.
Microtubule architecture in wild type and αTAT mutants.
| Genotype | Transgene | n | MTs/section | MT length (µm)§ | pf number† |
|---|---|---|---|---|---|
| wild typea | none | 3 | 62 ± 7 | 20.1 ± 0.4 | wild type |
| mec-12 | none | 3 | 0±0 | 0±0 | n/a |
| atat-2 | none | 3 | 37 ± 17 | 15±2.4 | wild type |
| mec-17 | none | 3 | 8.8 ± 1.6 | 7.5±2.8 | variable |
| mec-17;atat-2 | none | 3 | 7.1 ± 0.6 | 2.29±0.06 | variable |
n = the number of TRNs serially sectioned and examined for each genotype. Each serial section data set consisted of at least 60 ultrathin sections; individual sections were 50-nm thick.
a
Data derived from an HPF-FS serial section dataset reported in Ref. 22.
§
Average microtubule length was calculated using the formula L = 2Na/T where L is average microtubule length, N is the average number of microtubules per section, a is the length of the series, and T is the total number of microtubule terminations [5].
†
wild type = most microtubules have 15 protofilaments, though rare 11-pf microtubules are present; variable = microtubules have 9 to 16 protofilaments.