Figure 3. Screening for JAK-STAT modulating genes by qPCR. (A) Heat map showing the level of IFN-γ induced GBP1 mRNA and OSM induced SOCS3 mRNAs expressed following knock down of the indicated genes. Numbers represent the fold change relative to controls. Colors represent statistically significant increases (reds) or decreases (blues) in expression with dark red/blue p < 0.001 and light red/blue p < 0.05. Genes have been grouped according to phenotype and are discussed in the main text. (B and C) Graphs representing the interactions of genes falling into predicted endocytosis and protein stability (B) or chromatin modifier (C) ontologies. Error bars show standard error. *p < 0.05, **p < 0.01, ***p < 0.001.