Table 1. Primary sequence comparison of regions of the α-, β-, andγ-subunits with the amino acid residues that have been identified in the mgi complementation group.

The mutations in the genes encoding the α-, β-, and γ-subunits (ATP1, ATP2, and ATP3) are shown corresponding to the mgi complementation group identified from K. lactis, S. cerevisae, and T. brucei (8,12–14). The altered residues are indicated in bold and underlined. The mutations ATP1–75, ATP3–1, and ATP3–5 were identified from S. cerevisiae and classified in those studies as dominant mutations. The abbreviation used for T. brucei is T.B.

α–subunit		β–subunit
E.coli	5 8–NRYAIALNLERDSVG–7 2	E.coli	3 8 8–KLWARARKIQRFLS – 4 01
S.cerevisiae	6 0–GVKGMALNLEPGQVG–7 4	S.cerevisiae	401–KLTVERARKIQRFLS–419
Bovine	5 8–GLKGMSLNLEPDNVG–7 2	Bovine	401–KLTVSRARKIQRFLS–419
K. lactis	9 8–GVKGMALNLEPGQVG–112	K. lactis	428–KLTVERARKIQRFLS–442
ATPl–75	6 0–GVKGMALILEPGQVG–7 4	atp2–l	428–KLTVERAGKIQRFLS–442
		atp2–3	428–KLTVERAIKIQRFLS–442
α–subunit		atp2–6	428–KLTVERATKIQRFLS–442
E.coli	27 6–LLRRPPGREAFPGDV–290	atp2–7	428–KLTVERAVKIQRFLS–442
S.cerevisiae	286–MLRRPPGREAYPGDV–300	atp2–8	428–KLTVERAKKIQRFLS–442
Bovine	2 84–LLRRPPGREAYPGDV–2 9 8
K. lactis	324–LLRRPPGREAYPGDV–338	β–subunit
atpl–2	324–LLRRPPGREVYPGDV–338	E. coli	259–LGRMPSAVGYQPTLA–273
atpl–3	324–LLRRPPGRETYPGDV–338	S. cerevisiae	272–LGRMPSAVGYQPTLA–286
atpl–6	3 2 4–LLRRLPGREAYPGDV–338	Bovine	272–LGRMPSAVGYQPTLA–286
		K. Lactis	299–LGRMPSAVGYQPTLA–313
α–subunit		atp2–9	299–LGRMPSAFGYQPTLA–313
E.coli	400–YRELAAFSQFASDLD–415
S.cerevisiae	399–YREVAAFAQFGSDLD–413	γ–subunit
Bovine	397–YREVAAFAQFGSDLD–411	E.coli	264–VYNKARQASITQELTEI–2 80
K. lactis	437–YREVAAFAQFGSDLD–451	S.cerevisiae	254–LYNRTRQAVITNELVDI–270
atpl–1	437–YREVAASAQFGSDLD–451	Bovine	249–TFNRTRQAVITKELIEI–2 65
atpl–5	437–YREVAALAQFGSDLD–451	K. lactis	265–LYNRTRQAVITNELVDI–2 81
		atp3–l/ATP3–5	265–LYNRTRQAVIANELVDI–281
		atp3–2/ATP3–l	265–LYNRTRQAVITNELVDT–281
		T. Brucei	262–LYNKTRQFGITAALIEI–278
		T.B. atp3–3	262–PYNKTRQFGITAALIEI–278