Figure 2.

Control experiments. (A) Specific activity of palmitic or oleic acid oxidiation in C. elegans wild type animals. (B) β-oxidation of palmitic acid in live and heat-killed worms. L4 worms were incubated with tritiated oleic acid complexed to BSA for one hour, and the amount of tritiated water generated by oxidation was determined, normalized to protein levels, and expressed relative to control levels. For the boiling experiments, worms were boiled in 5 mL S-basal for 15 min at 95°C. (C) β-oxidation of oleic acid in controls vs. azide-treated worms. Azide to 10mM was added to the samples just after addition of assay mix. Average ± SEM is shown; n indicates the number of determinations. Asterisks indicate statistical significance (***: p < 0.0001).