Table 1. Different assay systems for analyzing thermotaxis.
| Experimental conditions |
|
Evaluations * |
References |
||
|---|---|---|---|---|---|
| Thermal gradients | Assay times | ||||
|
Spacial |
nonlinear |
0.5°C/cm–3.5°C/cma |
1–2 h |
track |
5, 10, 11, 12, 32, 34, 35, 45, 59, 63, 64 |
| |
linear |
0.5°C/cm |
1 h |
distribution |
5, 9, 12, 26, 32, 37, 45 |
| |
(~0.5°C/cm) |
0.5°C/cm |
to 1 h (time course) |
distribution |
18 |
| |
|
0.5°C/cm |
1 h |
run duration |
24 |
| |
|
0.5°C/cm |
30 min |
run duration |
61 |
| |
|
0.5°C/cm |
30 min |
IT duration |
24 |
| |
|
0.5°C/cm |
25 min |
IT temperature |
18 |
| |
|
0.4°C/cm |
23 min |
IT temperature |
15 |
| |
linear |
1.15°C/cm |
to 1 h (time course), 8 h |
distribution |
17 |
| |
(~1°C/cm) |
1.0°C/cm |
35 min |
run duration |
13 |
| |
|
0.8°C/cm |
30 min |
IT duration |
24 |
| |
|
1.1°C/cm |
30 min |
IT temperature |
39 |
| |
|
1.0°C/cm |
25 min |
IT temperature |
18 |
| |
|
0.9°C/cm |
35 min |
IT length and temperature |
33 |
| |
linear |
1.4°C/cm |
1 h |
distribution |
16 |
| |
(1.4°C/cm) |
1.4°C/cm |
10 min |
instantaneous velocities |
16 |
| |
linear (various) |
0.2°C/cm, 0.4°C/cm, 0.6°C/cm, 0.8°C/cm, 1.0°C/cm, 1.2°C/cm |
1 h |
distribution |
19 |
| |
|
0.3°C/cm, 0.5°C/cm, 1°C/cm, 1.5°C/cm |
10 min |
distribution |
20 |
| |
|
0.40°C/cm, 0.75°C/cm, 2°C/cm |
23 min |
IT amplitude |
15 |
|
Temporal |
crawling |
± 0.5°C/min |
5 min |
run duration |
15 |
| |
swimming |
± 4°C/min |
30 sec |
turning rate |
15 |
| |
|
± 2°C/min |
15 minc |
turning rate |
36 |
| various stimulib ex. from 0.4°C/min to 6.4°C/min |
5–15 mind | turning rate | 61 | ||
*
Values were calculated from these ‘evaluations’ by respective equations in some articles.
a Cassata et al., Genesis, 2000.
b Sine-wave, upstep and downstep.
c repeated up (1min) and down (1min).
d involved the presentation of multiple stimulus waveforms.