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Expression of transgenic CtBP restores repression of intergenic transcripts. ctbp+/− mutant flies were crossed to either FLAG-dCtBP transgenic flies or wild-type flies. Embryos were harvested at 3 h post-egg-lay, either left untreated or heat-shocked to induced CtBP expression, and RNA was isolated at 15 h and subjected to RT-PCR with the primers shown above the lanes. Genotypes and treatments are shown above each lane.
