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CtBP levels control PHO and Pc DNA binding and histone modifications. Wild-type and ctbp+/− embryos were harvested at 14 h and processed for ChIP assay with antibodies against PHO, Pc, H3 trimethyl-lysine 27, or H3 acetyl-lysine 9 followed by PCR with primers to the PRED (A), scr (B), or engrailed (C) PRE sequences.
