Expression of a CtBP transgene restores PHO and Pc DNA binding as well as methylation of H3 lysine 27. Wild-type and ctbp+/− embryos were harvested at 3 h, either left untreated or heat-shocked to induce CtBP expression, then processed for ChIP assay with antibodies against PHO, Pc, H3 methyl-lysine 27, or H3 acetyl-lysine 9 followed by PCR with primers to the PRED (A), scr (B), or engrailed (C) PRE sequences.