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. Author manuscript; available in PMC: 2013 Jun 3.
Published in final edited form as: J Cell Biochem. 2010 May;110(1):62–69. doi: 10.1002/jcb.22487

Fig. 5.

Fig. 5

Expression of a CtBP transgene restores PHO and Pc DNA binding as well as methylation of H3 lysine 27. Wild-type and ctbp+/− embryos were harvested at 3 h, either left untreated or heat-shocked to induce CtBP expression, then processed for ChIP assay with antibodies against PHO, Pc, H3 methyl-lysine 27, or H3 acetyl-lysine 9 followed by PCR with primers to the PRED (A), scr (B), or engrailed (C) PRE sequences.