TABLE I.
Primers used for RT-PCR analyses to detect intergenic transcripts across PRE-D, scr and en PRE sequences.
| Primer name | Primer sequence |
|---|---|
| PRED sense 1F | CAGCCGTGCGGTATGGAGAG |
| PRED sense 1R | CTTGCAAAAGCGGCTATGAAAAG |
| PRED sense 2F | ATAAAACCCCAGTGCGAAATGC |
| PRED sense 2R | TGCGCGTAGTCTTATCTGTATCTCG |
| PRED sense 3F | TAAAGCGAGAGCGATCCGAGC |
| PRED sense 3R | AAACGGCCATTACGAACGACAG |
| PRED sense 6F | TTCGGGCTTGTATTCGTGTTTTG |
| PRED sense 6R | TTACGGCCCTTTTATAGATGTTGC |
| PRED sense 5F | TACGCACGTCAGACTTGGAATAGC |
| PRED sense 5R | CAAGCCCGAAAAAGAAGAAGAAGC |
| PRED sense 4F | AAAACGGCCATTACGAACGACAG |
| PRED sense 4R | GACGTGCGTAAGAGCGAGATACAG |
| PRED antisense 1F | CGGCCCTTTTATAGATGTTGCAAC |
| PRED antisense 1R | CCGCCGCTTCTTCTTCTTTTTC |
| PRED antisense 2F | ATAAAACCCCAGTGCGAAATGC |
| PRED antisense 2R | TGCGCGTAGTCTTATCTGTATCTCG |
| PRED antisense 3F | GCAAACATGGGCAAACACAACC |
| PRED antisense 3R | CAGCTCCGTCGCCATAACTGTC |
| PRED antisense 4F | CGTAATGGCCGTTTTAAGTGCG |
| PRED antisense 4R | TAAGCAAACATGGGCAAACACAAC |
| PRED antisense 5F | GCCCAGTGAAAATTTGGCAGC |
| PRED antisense 5R | ACAGCCGTGCGGTATGGAGAG |
| Scr 1F | AATCGGTCGAATTATTTAGCAAC |
| Scr 1R | ACTTCATCGGCAGTCTTGGAG |
| Scr 2F | GTAATTTTTATTTTTTGTTGC |
| Scr 2R | GCCCCTGCTTTCTACCATCTCC |
| Scr 3F | GAGAGGCCTTTGATTGTGTGG |
| Scr 3R | TCGACCGATTATGGAAAACTG |
| Scr4F | ATGCAGCTGGGAAATCGTTGG |
| Scr 4R | GGCCTCTCCTGGTTTATCTTTG |
| Scr 5F | AATGGCTGATTTGGGTTCTCTG |
| Scr 5R | ATAGGCCACCGGGTAACATTTTG |
| Engrailed 1F | ATCGTGTATTTAGCGTATTTTTG |
| Engrailed 1R | CAACTTTATCGACACCACCTTTAG |
| Engrailed 4F | CAGCATGCGCATAATAAAGTC |
| Engrailed 4R | TTTCGCCGGCTCACTCACTC |
| Engrailed 5F | GTTCGCATGGGGCAGGTGAC |
| Engrailed 5R | TAGCTGGCGAAGTGTGTGCG |
| Engrailed 6F | CTCGCTCGCTCGCACACAC |
| Engrailed 6R | TGACTACTTCGGAATCGCAGC |