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. 2012 Apr 1;1(2):134–138. doi: 10.4161/worm.19501

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Copyright © 2012 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name worm-1-134-g2.jpg — Figure 2.C. elegans epidermal wound responses. (A) Epidermal GCaMP fluorescence elevation after femtosecond laser wounding. Pcol-19-GCaMP3(juIs319). Lateral views of adult epidermis in mid-body before, immediately after and 1 h after laser wounding; x marks site of laser wound. Spinning disk confocal, intensity code; scale, 10 μm. (B) Needle wounding triggers actin assembly around the wound site. Pcol-19-GFP::Moesin (juIs352) labels actin filaments in adult epidermis. At 24 h an autofluorescent “scar” (red) is visible at the wound site and the actin structures have disappeared. x marks site of needle wound; laser scanning confocal images; scale, 10 μm. C, Graphical summary of mutant or RNAi phenotypes of genes implicated in C. elegans wound-induced actin dynamics.