Skip to main content
. 2013 Apr 23;77(5):350–360. doi: 10.1111/sji.12041

Figure 1.

Figure 1

Low-concentration IL-1ß (IL-1ßlow) in combination with rat recombinant (r) or calf thymus-extracted (t)HMGB1 induces PGE2 and cytokine production. SFs were cultured in the presence of IL-1ß and HMGB1 separately or in complex. PGE2 production and cytokine release were measured in supernatants after 24 h of stimulation. (A) PGE2 production obtained when using rHMGB1 (n = 12) (B) PGE2 production obtained when using tHMGB1 (n = 4). (C) IL-6 release (rHMGB1) (n = 3) D) IL-8 release (rHMGB1) (n = 3). (A–D) results are expressed as per cent of the IL-1ßlow/r/tHMGB1 response to account for interpatient variability (mean ± SEM). PGE2 was measured by EIA and cytokines by CBA. P values were calculated by parametric ANOVA (Tukey–Kramer post hoc test).