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. 2013 May 14;110(22):9096–9099. doi: 10.1073/pnas.1305187110

Fig. 1.

Fig. 1.

CD134 interacts with HHV-6B gH/gLgQ1/gQ2 and is expressed on HHV-6B permissive cells. (A) Purified HHV-6A- or HHV-6B gHFc/gL/gQ1/gQ2 complex was incubated with the lysates from Molt-3 cells whose cell surface was labeled with EZ-link sulfo-NHS-Lc-Biotin. The complex and its binding proteins were immunoblotted and detected with streptavidin, anti-CD46, or anti-CD134 antibody. (B) CD134 or CD46 expression on HHV-6B permissive and nonpermissive cells. HHV-6B permissive (Molt-3 and MT4) or nonpermissive (SupT1, JJhan, and HSB-2) cells were stained with anti-CD46 or CD134 antibody, followed by staining with secondary antibody for FACS analysis. Gray shading, isotype control. (C) Down-regulation of CD134 expression from the cell surface after HHV-6B infection. CD134 or CD46 expression on the surface of HHV-6B–infected Molt-3 cells was determined as described in B. Gray shading, isotype control; dark gray line, mock infection; light gray line, 2 h or 48 h postinfection (p.i.).