Table 1.

FRAP data analysis

GFP fusions*	Parameters†	Mobile fraction‡	t1/2 (s)§
S2168-GFP	t = 120 s, n = 13	0.35	45.95
	t = 320 s, n = 11	0.47	69.40
S2168-GFP + irsS2168	t = 60 s, n = 24	0.93	12.25
	t = 180 s, n = 14	1.03	14.51
irsS2168-GFP	t = 30 s, n = 22	0.97	5.77
	t = 180 s, n = 10	0.88	8.07
irsS2168-GFP with DNP	t = 30 s, n = 17	0.87	3.69

^*Fusions expressed in each experiment.

^†Experimental parameters. t, duration of the FRAP experiment in seconds; n, number of individual cells photobleached.

^‡The fraction of protein assessed as mobile based on the plateau of the FRAP recovery curve.

^§The half-life of fluorescence recovery calculated from the FRAP recovery curves (details in Experimental Procedures).