Table 2.
Strains, phage, and plasmids
| Genotype and relevant features | Source | |
| Phages | ||
| λcamΔ(SR) | λb515 b519 att::Tn903 cI857 nin5 Δ(SR) bor::camr | Laboratory stock |
| λcam(S68-gfp RamRzRz1)21 | λb515 b519 att::Tn903 cI857 nin5 Δ(SRRzRz1):: (S68-gfp RamRzRz1)21 bor::camr | This study |
| E. coli strains (all K–12) | ||
| MG1655 | ilvG rfb50 rph1 | 25 |
| MG1655lacIq1 tonA::Tn10 | 13 | |
| MDS12 | MG1655 with 12 deletions, totaling 376,180 nt, including all cryptic prophages | 36 |
| MDS12 ΔtonA | 4 | |
| MDS12 tonA::Tn10 | 30 | |
| MDS12 tonA::Tn10[λcamΔ(SR)] | Lysogen carrying prophage λcamΔ(SR) | This study |
| MDS12ΔtonA[λcam(S68-gfp RamRzRz1)21] | Lysogen carrying prophage λcam(S68-gfp RamRzRz1)21 | This study |
| MDS12ΔtonA(λS2168) | Lysogen carrying prophage λS2168 | This study |
| LE392 tonA::Tn10 | hsdR574(rK−mK+) supE44 supF58 Δ(lacIZY)6 galK2 galT22 metB1 trpR55 tonA::Tn10 | 28 |
| Plasmids | ||
| pTP2 | pBR322 origin, pR′ promoter, (S68/R/Rz/Rz1)21 | 13 |
| pR′S2168 | pTP2 (Ram/Rzam/Rz1am)21 | |
| pS2168ΔTMD1 | pTP2 with the codons encoding TMD1 of S2168 deleted, (Ram/Rzam/Rz1am)21 | 4 |
| pirsS2168 | pR′S2168 except encoding an S2168 with the irs tag (residues RYIRS) fused to the N terminus | 4 |
| pQ | pSC101 origin with the λ Q late-activator gene under the control of the Plac/ara-1 promoter | 26 |
| pRE | pJF118EH with lacIQ and Ptac replaced by pR′ promoter | 13 |
| pGFP | pRE with gfp gene inserted downstream of the pR′ promoter | Laboratory stock |
| pS2168-GFP | pR′S2168 with codons encoding linker sequence Pro and Gly (CCCGGG) and the gfp gene without Met1 (AUG) inserted between the last codon (GAA) of S2168 and the stop codon (UAA) | This study |
| pS2168ΔTMD1-GFP | pS2168ΔTMD1 with the linker and gfp same as in pS2168-GFP | This study |
| pirsS2168-GFP | pirsS2168 with the linker and gfp same as in pS2168-GFP | This study |
| pS2168-GFP (RamRzRz1)21 | pS2168-GFP with WT Rz21and Rz121 | This study |