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Subsets of blast colony cells vary in their capacity to produce mast cells and basophils
| Fraction | No. of cells cultured per well | No. of assay wells | % wells with mast cells | % wells with basophils |
| Kit+ Sca1− | 2,000 | 12 | 0 | 18 |
| Kit+ Sca1+ | 580 | 12 | 17 | 17 |
| Kit− Sca1− | 2,000 | 23 | 13 | 22 |
| Kit− Sca1+ | 2,000 | 25 | 68 | 82 |
Fractionated blast colony cells were cultured for 3 wk with SCF plus IL-3. Pooled data from two separate experiments are presented. In some cases, negative wells contained no living cells.
