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. 2013 May 13;110(22):9084–9089. doi: 10.1073/pnas.1300595110

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Fig. 4. — Identification of CpaA as an additional c-di-AMP target protein. (A) Schematic representation of the predicted K⁺ or Na⁺ antiporter CpaA (SAUSA300_0911), containing an N-terminal transmembrane (yellow) and cytoplasmically located RCK_N (blue) and RCK_C (orange) domains. (B) DRaCALAs with ³²P-labeled c-di-AMP and E. coli extracts prepared from the vector control strain (pET28b) or strains overproducing His-CpaA_SA-402-614 (RCK_N and RCK_C) or His-CpaA_SA-513-614 (RCK_C). Cold c-di-AMP was added as a competitor where indicated.