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Dna2 E675A shows DNA-dependent ATPase activity that is stimulated by RPA. (A) Rate of ATP hydrolysis and its dependence on the DNA concentration. The reactions contained Dna2 E675A (4 nM), ATP (1 mM), RPA where indicated (150 nM), and the indicated concentrations of poly(dT). (B) Apparent ATP turnover number and its dependence on various DNA structures (1 μM nucleotides). The reactions contained Dna2 E675A (3 nM) and ATP (1 mM). Error bars, SE, n = 2.
