Fig. 4.

lym2-1 mutants have increased susceptibility to B. cinerea but not C. higginsianum and Pto DC3000. B. cinerea (A) or C. higginsianum (B) was drop-inoculated on leaves of Col-0 or lym2-1 plants. Lesion diameter measured after 3 d for B. cinerea (D) or 5 d for C. higginsianum (D). (C) Col-0 and lym2-1 were spray-inoculated with Pto DC3000 and bacterial growth assessed as colony counts (log₁₀ cfu/g) determined 2 dpi from pooled extracts (2 plants per count). Error bars indicate SD. Asterisks indicate statistical significance: ***P < 0.001. (E) Trypan blue-stained leaves infected with B. cinerea showed that at 24 and 36 hpi, infection sites appeared the same in Col-0 and lym2-1, showing a similar degree of cell death in the epidermis (24 h) and mesophyll (36 h). At 48 hpi, mesophyll cell death in Col-0 leaves was restricted to the site of infection (solid line), but in lym2-1 leaves, it was observed beyond this boundary (dotted line). [Scale bars, 0.5 cm (D) and 100 μm (E)].