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mRNA stabilization by a 5′-terminal stem loop in B. subtilis cells lacking RppH. Total RNA was extracted at time intervals after rifampicin addition to B. subtilis ∆rppH cells that contained a plasmid encoding mini yhxA-glpP mRNA (2G) or a sequence variant (2A, 2C, or 2U), with (+hp) or without (−hp) a 5′-terminal stem loop, and mRNA half-lives were compared. Error bars correspond to SDs.
