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Differential labeling of old pole and growth pole by FM 4-64. (A) FM 4-64 labels older halves of cells (single arrowhead), both ends of a cell (double arrowhead), and S-shaped cell pairs (triple arrowheads). (Scale bar, 2 μm.) Close-ups of FM 4-64 labeling: (B) more intense labeling of old pole; brackets indicate unlabeled growth poles; (C) both poles labeled in a cell with midcell constriction; and (D) S-shaped cells just post cell division. White dots show the cell outlines.
