Fig. 1.

Size-exclusion chromatography (SEC) of the BUNV NP–RNA complex with RNase pretreatment. The E. coli-expressed BUNV NP (0.2 mg) pretreated with RNase (0.2 mg/mL at 16 °C for 12 h) was injected onto a Superdex 200 HR 10/30 column. The absorbance at 260 and 280 nm are indicated in red and blue, respectively. The retention volume for the major peak was 14.5 mL. The retention volumes for the molecular-weight standards are shown above. SDS/PAGE analysis of the SEC elution fractions corresponding to the peaks is shown with standard protein markers.