Table 1.

Genes up-regulated at least 1.4-fold by p-coumaric acid as identified by three independent methods of RNA-seq expression analysis

Gene name	Fold change	Accession no.
Antimicrobial genes
Drug- metabolizing genes
Phase I
Abaecin	1.90	NP_001011617
CYP6AS2	2.70	XP_395085
CYP6AS3	3.11	XP_001122413
CYP6AS4	2.60	XP_395671
CYP6AS5	2.55	NP_001035324
CYP6BD1	1.92	XP_623955
CYP9Q3	2.55	XP_392001
β-Esterase, E-class	1.47	XP_392696
Phase II
α-Glutamyltransferase 1	1.50	XP_393584
UGT334C1 glucosyltransferase	1.46	XP_001123301
UGT318A3 glucosyltransferase	2.26	XP_396494
UGT332F1 glucosyltransferase	1.81	XP_392727
Phase III
Multidrug resistance-associated protein 4	1.60	XP_623460
Multidrug resistance-associated protein 1	1.80	XP_003249371
Other genes
FABP-like protein	1.58	NP_001011636
Inorganic phosphate cotransporter-like protein	1.60	XP_393759
Similar to CG13424 (genome assembly nucleotide accession)	1.59	NW_003377962
Protein lethal(2)essential for life-like	1.92	XP_393575
Hypothetical protein LOC408807	3.69	XP_397526
Sarcoplasmic calcium-binding protein 2	2.43	XP_003249222
Hypothetical protein LOC552202	1.55	XP_624582
Venom acid phosphatase	1.71	NP_001013377
Luciferin 4-monooxygenase-like	2.81	XP_001122105

Dataset S1 provides Cuffdiff analysis of differential gene expression in the midguts of honey bees fed with p-coumaric acid-containing sugar candy (CoA) vs “bee candy” (CK); Dataset S2 provides DESeq analysis of differential gene expression in the midguts of honey bees fed with p-coumaric acid-containing sugar candy (CoA) vs “bee candy” (CK); Dataset S3 provides edgeR analysis of differential gene expression in the midguts of honey bees fed with p-coumaric acid-containing sugar candy (CoA) vs “bee candy” (CK). See Fig. S2 for depiction of intersecting sets of genes up-regulated by p-coumaric acid as determined by RNA-seq of genes and identified by all three analytical methods.