Fig. 2.

Hypoxia decreases SCD1 flux and increases fatty acid import. (A) Schematic of oleate (C18:1) synthesis. (B) Labeling patterns of C18:0 and C18:1 from MDA-MB-468 cells grown in [U-¹³C]glucose and [U-¹³C]glutamine, in normoxia and hypoxia (1% O₂) for 72 h. (C) Desaturation index (C18:1/C18:0) in normoxia and hypoxia (1% O₂ for MDA-MB-468 and HeLa cells and 0.5% for A549 cells). (D and E) Percentage of import of C18:0 (D) and C18:1 (E) pools in normoxia and hypoxia, as measured by fatty acid labeling for 72 h, from [U-¹³C]glucose and [U-¹³C]glutamine. All data are means ± SD of n = 3. *P < 0.05; **P < 0.01 (two-tailed t test).