Fig. 3.

A. muciniphila colonization restored gut barrier function and increased intestinal endocannabinoids in diet-induced obese mice. (A) Ileum 2-PG, 2-OG, and 2-AG (expressed as percentage of the control) (n = 10). (B) Thickness of the mucus layer measured by histological analyses After alcian blue staining (n = 7–8). (C) Representative alcian blue images that were used for mucus layer thickness measurements. M, mucosa; IM, inner mucus layer. (Scale bars, 40 µm.) Data are shown as means ± SEM. Data with different superscript letters are significantly different (P < 0.05) according to post hoc ANOVA one-way statistical analysis.