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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Anal Biochem. 2012 Dec 5;434(1):181–186. doi: 10.1016/j.ab.2012.11.022

Figure 3.

Figure 3

Addition of the osmolytes glycerol, D-sorbitol, L-proline, and D-sucrose to the cultivation medium restores β-lactamase activity in a dose-dependent manner in extracts of E. coli MG1655 ΔampCΔhsdR expressing β-lactamase -Im7 I22V-β-lactamase. Absolute enzymatic activity is expressed as the rate of hydrolysis of nitrocefin (determined by measuring absorbance at 486 nm over time), adjusted to the cell OD of the cell extract. Mean values ± standard deviations are shown for independent triplicate experiments except for sucrose in which data are from one experiment